SURVIVABILITY & SHELF LIFE

GROWTH

Aerobic to microaerophilic. On solid media (Glucose Yeast Extract Agar) forms smooth, circular, convex, opaque white colonies after incubation at 37°C for 2-3 days. Grow at temperature of 30-60°C, optimally at 37°C and at pH range 4.4 – 10.6, optimally 6-7. Form L(+) lactic acid homofermentatively. Spores are acid fast and thermostable in nature and withstand heating at 90°C for 30 minutes.

(METCHNIKOFF-BIOSYSTEMS PVT.LTD., PROBIOTIC –LSP)

Some of the characteristics listed for Lactobacillus sporogenes (synonym Bacillus coagulans) in the Seventh Edition of Bergey’s Manual and elsewhere (10,30,31) are “Gram positive, spore forming rods, 0.9 by 3.0 to 5.0 micron size, aerobic to microaerophillic, produce L(+) lactic acid (dextrorotatory) homofermentatively”. They have a growth temperature range of 34°C to 50°C and an optimum pH range of from 5.5 to 6.5.

(LACTOBACILLUS SPOROGENES AN ADVANCEMENT IN LACTOBACILLUS THERAPY – DR. A.B. GHANDI)

STORAGE AND STABILITY

Storage of PROBIOTIC-LSP (Lactobacillus sporogenes powder) by Refrigeration, Lyophilization or under the atmosphere of Nitrogen is not at all necessary. Preserve powder in tight container and keep in dry cool place. PROBIOTIC-LSP (Lactobacillus sporogenes powder) is stable for more than 2 years at room temperature and retains 90-95% activity over this period. (METCHNIKOFF – BIOSYSTEMS PVT. LTD., PROBIOTIC – LSP)

LACTOBACILLUS SPORES (Lactobacillus sporogenes) are quite stable and do not decrease in numbers during process of manufacture, handling and upon storage for long period at room temperature. They are stable to heat, gastric acidity, bile and many other deleterious chemicals. The spores, after germination, reproduce by binary fission in intestines, increasing stationary population of the bacteria with gradual production of lactic acid.

(PROBIOTICS – A Sporiferous Preparation of Lactobacillus Sporogenes – as LACTOBACILLUS SPORES POWDER).

Lactobacillus sporogenes is now noticed with keen interest as a new and powerful species of lactobacilli having many advantages over ordinary lactobacilli such as stability in terms of viabile bacterial count, survival in gastric acidity, favorable proliferating rate in the intestinal tract and superior L+ lactic acid (dextrorotatory) producing capacity (29).

(LACTOBACILLUS SPOROGENES AN ADVANCEMENT IN LACTOBACILLUS THERAPY – DR. A.B. GHANDI)